Coding

Part:BBa_K4192010:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


cdg gene from Pseudomonas fluorescens str.2P24


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BamHI site found at 254
    Illegal XhoI site found at 352
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 187
    Illegal AgeI site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 772


Design Notes

It is worth noting that the low expression of cdg may promote the movement of bacteria, thereby reducing the production of biofilm and bringing other results. It can play a better role when co-expressed with other regulatory factors such as gacA (BBa_K4192011).


Source

It comes from the genome of Pseudomonas fluorescens 2P24.

References